Download MendeleyGlucosyltransferase TeGSS from Thermosynechococcus elongatus produces an alpha-1,2-glucan.
Qu, X., An, Q., Sayed, H., Cui, L., Mayo, K.H., Su, J.Y.(2024) Int J Biol Macromol 280: 136152-136152
- PubMed: 39357710
- DOI: https://doi.org/10.1016/j.ijbiomac.2024.136152
- Primary Citation of Related Structures:
8X3Q, 8X3U - PubMed Abstract:
Here, we enzymatically produced a novel α-1,2-glucan, glucosylsucrose, that has a chemical structure significantly different from that of other glucans. This structural difference suggests its potential to modulate new physiological activities compared to known glucans. The enzyme TeGSS catalyzes the synthesis of this α-1,2-glucan from sucrose and UDP-glucose (UDPG). Using NMR spectroscopy, we elucidated the chemical structures of TeGSS-synthesized glucosylsucrose tri-, tetra-, and penta-saccharides in which the monosaccharide units are linked by α-1,2-glycosidic bonds. We also report the crystal structures of TeGSS co-crystallized with UDP and glucosylsucrose tri- and tetra-saccharides. Site-directed mutagenesis of residues in and around the TeGSS catalytic center has allowed us to propose a concerted S N i mechanism of action. Finally, we developed an enzyme-coupled reaction involving TeGSS and SuSyAc that allows production of UDPG for the synthesis of α-1,2-glucan.
Organizational Affiliation:
Engineering Research Center of Glycoconjugates Ministry of Education, Jilin Provincial Key Laboratory of Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, China.
